Effect of aurora inhibitor VX-680 on proliferation and apoptosis of CML cells.
10.7534/j.issn.1009-2137.2014.06.008
- Author:
Yue YIN
1
;
Hui-Yan SUN
2
;
Xiao-Lin LI
3
;
Feng-Jun XIAO
2
;
Li-Sheng WANG
4
Author Information
1. Department of Hematology, Peking University First Hospital, Beijing 100034, China.
2. Department of Experimental Hematology, Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
3. Department of Hematology, Xuzhou Central Hospital of Jiangsu Province, Xuzhou 221009, Jiangsu Province, China.
4. Department of Experimental Hematology, Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China. E-mail:wangls@bmi.ac.cn.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
Aurora Kinase A;
antagonists & inhibitors;
Cell Line, Tumor;
Cell Proliferation;
drug effects;
Humans;
Leukemia, Myelogenous, Chronic, BCR-ABL Positive;
pathology;
Piperazines;
pharmacology;
Protein Kinase Inhibitors;
pharmacology
- From:
Journal of Experimental Hematology
2014;22(6):1540-1544
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to explore the effect of VX-680, an aurora inhibitor, on proliferation and apoptosis of K562, KCL22 cell lines and CD34⁺ cells from chronic myeloid leukemia (CML) patients in vitro. The proliferation of K562 and KCL22 cell was detected by CCK-8 method. Apoptosis of cells was detected by Annexin V-PI labeling and flow cytometry. The colony forming ability of bone marrow CD34⁺ cells derived from CML patients and donors was determined by the colony forming test. The results showed that the treatment of K562, KCL22 and CML CD34⁺ cells with VX-680 of 20-100 nmol/L for 3 days could obviously inhibit the cell proliferation in a concentration-dependent manner (P < 0.01). VX-680 treatment significantly induced apoptosis of K562 and KCL22 cells. Compared to bone marrow CD34⁺ cells derived from the healthy donors, the colony forming ability of CML CD34⁺ cells derived from bone marrow of CML patients was remarkably reduced (P < 0.01). It is concluded that VX-680, an aurora inhibitor, can inhibit the proliferation and induce apoptosis of CML cells in vitro.
