Efects of Inhibiting miR-155 Expression on the Proliferation and Apoptosis of Leukemia THP-1 Cells.
10.7534/j.issn.1009-2137.2014.06.010
- Author:
Hua XUE
1
;
Lu LIANG
2
;
Hui-Mei GUO
2
;
Luo-Ming HUA
2
;
Song-Ying ZHAO
2
;
Hong-Juan SHI
3
;
Jing-Yu ZHANG
3
;
Li SONG
3
Author Information
1. Department of Hematology, Affiliated Hospital of Hebei University, Baoding, Hebei Province 071000, China. E-mail: xh-xuehua@163.com.
2. Department of Hematology, Affiliated Hospital of Hebei University, Baoding, Hebei Province 071000, China.
3. Department of Internal Medicine, Yi County People's Hospital, Baoding 070000, Hebei Province, China.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
Cell Line, Tumor;
Cell Proliferation;
Flow Cytometry;
Gene Expression Regulation, Neoplastic;
Humans;
Leukemia;
genetics;
MicroRNAs;
genetics;
Phosphatidylinositol 3-Kinases;
RNA, Messenger;
RNA, Small Interfering;
Real-Time Polymerase Chain Reaction;
Reverse Transcriptase Polymerase Chain Reaction;
Signal Transduction;
Transfection
- From:
Journal of Experimental Hematology
2014;22(6):1550-1554
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study was to investigate the effects of miR-155 inhibitor transfection on the proliferation and apoptosis of THP-1 cells. The miR-155 inhibitor was transfected into THP-1 cells (THP-1I) by using X-treme GENE siRNA transfection reagent. Cells without transfection (THP-1C) and cells with negative transfection (THP-1IC) were used as controls. Quantitative real-time polymerase chain reaction (RT-PCR) was performed to detect the expression of miR-155 and relative expression of SHIP1 mRNA in the cells. Cell proliferation was assayed using CCK-8 method. Cell apoptosis were detected by flow cytometry. The expression of SHIP1, TAKT and pAKT in THP-1 cells were detected by Western blot. The results indicated that compared with THP-1C and THP-1IC, the expression of miR-155 in THP-1I cells was significantly reduced; miR-155 inhibition significantly increased apoptosis rate in THP-1 cells (P < 0.05) ; miR-155 inhibition in THP-1 cells caused no significant alteration in SHIP1 mRNA level but significantly increased its protein content, indicating some post-transcriptional modulations might exist underlying the modulation of miR-155 to SHIP1, the miR-155 caused significantly reduced protein level of pAKT (P < 0.05) without interfering TAKT protein content. It is concluded that the miR-155 inhibition may promote THP-1 cell apoptosis through increasing SHIP1 protein content and impairing its downstream PI3K/AKT signaling pathway. This study suggests that miR-155 inhibition may be a promising therapy strategy for treating acute myeloid leukemia (AML).
