MNPs-Fe₃O₄mediates malignant Hematolpoectic cell apoptosis.
10.7534/j.issn.1009-2137.2014.06.028
- Author:
Yu-Qiu LI
1
;
Bing WANG
1
;
Wen-Ce LI
1
;
Min GUO
1
;
Ying WANG
2
;
Yu-Jie GUO
3
;
Fu-Xu WANG
3
;
Shu-Peng WEN
3
;
Ling PAN
4
;
Xue-Jun ZHANG
3
Author Information
1. Hebei Provincial Blood Center, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China.
2. Department of Hematology, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China. E-mail: Wangying730@126.com.
3. Department of Hematology, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China.
4. Department of Hematology, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China. E-mail: Lingpan20002000@aliyun.com.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
Caspase 3;
Cell Cycle;
Cell Line, Tumor;
Ferric Compounds;
pharmacology;
Flow Cytometry;
Humans;
Magnetics;
Metal Nanoparticles;
administration & dosage
- From:
Journal of Experimental Hematology
2014;22(6):1649-1655
- CountryChina
- Language:English
-
Abstract:
This study was purposed to evaluate whether the safe concentration of magnetic nanoparticles of Fe₃O₄(MNPs-Fe₃O₄) for monocytes could induce the SKM-1 cell apoptosis. The average size and Zeta potential of MNPs-Fe₃O₄were determined by transmission electron microscopy and the Malvern Zetasizer 3000 HS, respectively. The cell viability after being exposed to MNPs-Fe₃O₄for 12, 24, 48, and 72 hours was detected by using cell count Kit-8. The cell apoptosis was evaluated by flow cytometry with Annexin V/PI double staining and Wright-Giemsa staining. The cell cycle was measured by flow cytometry. The levels of active caspase-3, survivin and bcl-rambo in cells treated with MNPs-Fe₃O₄and/or trolox for 48 hours were detected with Western blot. The results showed that the cell viability decreased in SKM-1 cells after exposure to 50 µmol/L and 100 µmol/L MNPs-Fe₃O₄(P < 0.05), but did not in monocytes (P > 0.05), compared with that of each non-MNPs-Fe₃O₄-treated group. This exposure also induced the SKM-1 cells to be arrested in G0/G1. Annexin V/PI staining assay showed that cell apoptotic rate induced by 100 µmol/L MNPs-Fe₃O₄was significantly high in SKM-1 cells while not so high in monocytes, and the pretreatment with trolox could attenuate the apoptosis. Moreover, the active caspase-3 increased in SKM-1 cells after the exposure to MNPs-Fe₃O₄, while that was not in monocytes, and the increased expression of BCL-rambo and the decreased expression of survivin involved in the process were also observed. It is concluded that MNPs-Fe₃O₄can induce the caspase 3-dependent SKM-1 cell apoptosis by increasing the BCL-rambo expression and decreasing the survivin expression, but this cytotoxic effect can not be observed in monocyte's.
