Anti-mouse CD122 antibody promotes the hematopoietic repopulating capacity of cord blood CD34⁺ cells in NOD/SCID mice.
10.7534/j.issn.1009-2137.2014.06.032
- Author:
Men-Yao SHENG
1
;
Hui SHI
1
;
Wen XING
1
;
Wen-Jun WANG
1
;
Xiao-Hui SI
1
;
Jie BAI
1
;
Wei-Ping YUAN
1
;
Yuan ZHOU
2
;
Feng-Chun YANG
1
Author Information
1. State Key Laboratory of Experimental Hematology, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Scinces & Peking Union Medical College, Tianjin 300020, China.
2. State Key Laboratory of Experimental Hematology, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Scinces & Peking Union Medical College, Tianjin 300020, China. E-mail: yuanzhou@ihcams.ac.cn.
- Publication Type:Journal Article
- MeSH:
Animals;
Antibodies;
immunology;
Antigens, CD34;
Bone Marrow;
Cord Blood Stem Cell Transplantation;
Fetal Blood;
immunology;
Hematopoietic Stem Cell Transplantation;
Hematopoietic System;
cytology;
immunology;
Humans;
Interleukin-2 Receptor beta Subunit;
immunology;
Killer Cells, Natural;
Mice;
Mice, Inbred NOD;
Mice, SCID;
Transplantation, Heterologous
- From:
Journal of Experimental Hematology
2014;22(6):1673-1677
- CountryChina
- Language:Chinese
-
Abstract:
The study was aimed to investigate the effect of anti-mouse CD122 antibody on the hematopoietic repopulating capacity of cord blood CD34⁺ cells in a humanized murine model-non obese diabetic/severe combined immunodeficiency (NOD/SCID) mice. After sublethal irradiation with γ-ray, NOD/SCID mice were intraperitoneally injected with 200 µg mouse isotype control antibody or anti-mouse CD122 antibody. Human cord blood CD34⁺ cells or phosphate-buffered saline (PBS) were injected via the tail vein at 6-8 hours later. Cohort of the mice injected with anti-mice CD122 antibody or control antibody alone were sacrificed at different time point (at week 2, 3, and 4 weeks) after the injection, and the percentage of NK cells in the peripheral blood was analyzed by flow cytometry. To evaluate the effect of anti-mouse CD122 antibody on the repopulating capacity of cord blood CD34⁺ cells in the recipient mice, phenotype analysis was performed in the bone marrow at 6 and 8 weeks after the transplantation. The results showed that the proportion of NK cells in the peripheral blood were (4.6 ± 0.6)% and (5.7 ± 1.7)% at week 2 and 3 after anti-CD122 antibody injection respectively,which decreased by 60%, compared with the mice injected with isotype control antibody. After 6 and 8 weeks of cord blood CD34⁺ cell transplantation,the percentage of human CD45⁺ in the bone marrow of the recipient mice treated with anti-mice CD122 antibody was (63.0 ± 12.2)% and (53.2 ± 16.3)%,respectively,which were dramatically higher than that in the mice treated with isotype control antibody (7.7 ± 3.6)% and (6.1 ± 2.4)%. Moreover,at 8 weeks after transplantation,human CD34⁺ cells appeared significantly in the recipients treated with anti-CD122 antibody. It is concluded that the anti-mouse CD122 antibody enhances the hematopoietic repopulating capacity of cord blood CD34⁺ cells in the NOD/SCID mice through decreasing the proportion of NK cells.
