Protective effect of HS-6101 on rhesus monkeys with severe hematopoietic acute radiation sickness.
10.7534/j.issn.1009-2137.2014.06.035
- Author:
Xing SHEN
1
;
Shuang XING
1
;
Guo-Lin XIONG
1
;
Ling XIE
1
;
Ling-Ling GUO
1
;
Zheng-Liang QIU
2
;
Chang-Hui GE
1
;
Chang-Liang DAI
3
;
Qing-Liang LUO
1
;
Xiao-Yu LIU
4
;
Zu-Yin YU
5
Author Information
1. Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
2. Expriment Animal Center, Academy of Military Medical Sciences, Beijing 100071, China.
3. Zhejiang Hisun Pharmaceutical Co.,Ltd. Taizhou 318000, Zhejiang Procince, China.
4. Zhejiang Hisun Pharmaceutical Co.,Ltd. Taizhou 318000, Zhejiang Procince, China. E-mail: xyliu@hisunpharm.com.
5. Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China. E-mail: yuzy79@yahoo.com.
- Publication Type:Journal Article
- MeSH:
Amifostine;
Animals;
Blood Cell Count;
Blood Platelets;
Bone Marrow;
Bone Marrow Cells;
Hematopoietic Stem Cells;
Hematopoietic System;
drug effects;
radiation effects;
Lipoproteins;
pharmacology;
Macaca mulatta;
Radiation Injuries;
drug therapy;
Survival Rate
- From:
Journal of Experimental Hematology
2014;22(6):1691-1697
- CountryChina
- Language:Chinese
-
Abstract:
This study was purposed to investigate the protective effects of lipoprotein HS-6101(6101) on rhesus monkey total body irradiated with 7.0 Gy ⁶⁰Coγ-ray. A total of 30 health adult rhesus monkeys were randomly divided into symptomatic therapy (ST), WR2721 and HS-6101 30, 90 and 270 mg/kg groups (n = 6), the rhesus monkeys of each groups were injected with physiological saline 0.3 ml/kg, WR-2721 30 mg/kg, or HS-6101 30, 90 and 270 µg/kg, respectively. All agents were once intramuscularly injected at 1 hr prior irradiation. General observation, peripheral blood cell counts, colony forming unite assay of bone marrow hemopoietic progenitor cells, and histopathological examination were performed. The results showed that animals in symptomatic therapy group begin to die on the 13(th) day and 4 animals died within 24 days, the average survival time was 18.2 ± 4.3 days; 2 animals in WR-2717 groups died on day 15.8 and day 18.5 post irradiation respectively. 1 animal in HS-6101 270 mg/kg group died on day 35.8, all other animals survived. Nadirs of peripheral blood white blood cells, neutrophils and platelets of animals in HS-6101 treatment groups were significantly higher than those in other 2 groups including ST and WR-2721 groups, and the hemopoietic recovery were also significantly speeding up(P < 0.05 and 0.01). In vitro results showed that HS-6101 obviously promoted 7.0 Gy ⁶⁰Coγ irradiated monkey's bone marrow mononuclear cells to form various hematopoietic progenitor cell colonies (P < 0.05 and 0.01) . Compared with symptomatic therapy and WR-2717 groups, bone marrow histopathological changes in HS-6101 treatment groups showed more active hemopoietic cell proliferation and higher density structure. It is concluded that HS-6101 90 µg/kg treatment can promote the bone marrow recovery of 7.0 Gy ⁶⁰Coγ irradiated monkey, alleviate their animal symptom, simplify the treatment measures and improve the animal survival rate. The HS-6101 shows remarkable radioprotective effects as compared with the currently internationally acknowledged radioprotectant of WR-2721.
