Effects of rhG-CSF Stimulation in vitro on the Adhesion and Polarization of Human CD4⁺T Lymphocytes.
10.7534/j.issn.1009-2137.2014.06.037
- Author:
Sha-Sha ZHAO
1
;
Zhen-Yang GU
1
;
Meng LI
1
;
Xiao-Li ZHAO
1
;
Lan LUO
1
;
Li-Xun GUAN
1
;
Li-Li WANG
1
;
Chun-Ji GAO
2
Author Information
1. Department of Hematology, Chinese PLA Medical College, Chinese PLA General Hospital, Beijing 100853, China.
2. Department of Hematology, Chinese PLA Medical College, Chinese PLA General Hospital, Beijing 100853, China. E-mail: gaochunji@medmail.com.cn.
- Publication Type:Journal Article
- MeSH:
Aged;
CD4-Positive T-Lymphocytes;
drug effects;
Cell Adhesion;
drug effects;
Cell Movement;
Cell Polarity;
drug effects;
Chemokine CXCL12;
Granulocyte Colony-Stimulating Factor;
pharmacology;
Humans;
In Vitro Techniques;
Intercellular Adhesion Molecule-1;
Leukocytes, Mononuclear;
Lymphocyte Activation;
Lymphocyte Function-Associated Antigen-1;
Middle Aged;
Recombinant Proteins
- From:
Journal of Experimental Hematology
2014;22(6):1702-1706
- CountryChina
- Language:Chinese
-
Abstract:
The adhesion and polarization of T lymphocytes involved in the adhesive interaction of lymphocyte function-associated antigen 1 (LFA-1) with its ligand intercellular adhesion molecule 1 (ICAM-1). This study was aimed to investigate the effects of recombinant human granulocyte colony-stimulating factor (rhG-CSF) stimulation in vitro on the adhesion and polarization of CD4⁺ T cells of healthy human in peripheral blood. The peripheral blood mononuclear cells were collected from 12 healthy volunteers. The CD4⁺ T cells were sorted by miniMACS. The sorted CD4⁺ T cells were incubated with rhG-CSF for 24 h, then the adhesion and polarization of CD4⁺ T cells activated by stroma cell-derived factor -1α (SDF-1α) and ICAM-1 were detected by ELISA and inverted microscope. The results showed that the percentage of adhesion CD4⁺T cells in the experimental group (rhG-CSF acting on the healthy adult volunteers) (61.9 ± 5.9)% was lower than that in the control group (healthy adult volunteers without rhG-CSF stimulation) (68.3 ± 7.3)% (P < 0.05). The percentage of polarized CD4⁺T cells in the experimental group (24.3 ± 4.3)% was also lower than that in control group (47.1 ± 5.1)% (P < 0.05). It is concluded that the adhesion and polarization of CD4⁺T lymphocytes can be inhibited after rhG-CSF stimulation.
