Kinetics of Cu crossing human erythrocyte membrane.
10.7534/j.issn.1009-2137.2014.06.038
- Author:
Zhu Ci Ren DUN
1
Author Information
1. Tibetan Traditional Medical College, Lasa 850000,Tibetan, China. E-mail: duncila@21cn.com.
- Publication Type:Journal Article
- MeSH:
Copper;
pharmacology;
Erythrocyte Membrane;
drug effects;
Humans;
Hydrogen-Ion Concentration;
Kinetics;
Temperature
- From:
Journal of Experimental Hematology
2014;22(6):1707-1710
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate various factors influencing the proceduction of Cu(II) crossing human erythrocyte membrane, including concentration of Cu²⁺, pH value of the medium, temperature and time of incubation, and to derive kinetic equation of Cu(II) crossing human erythrocyte membrane. Suspension red blood cells were incubated by Cu²⁺, then content of Cu²⁺ crossed human erythrocyte membrane was determined by atomic absorption spectrometry under various conditions after digestion. The results showed that content of Cu²⁺ crossed human erythrocyte membrane increased with the increase of extracellular Cu²⁺ and enhancement of incubation temperature, and the content of Cu²⁺ crossed human erythrocyte membrane showed a increasing tendency when pH reached to 6.2-7.4, and to maximum at pH 7.4, then gradually decreased at range of pH 7.4-9.2. It is concluded that the Cu²⁺ crossing human erythrocyte has been confirmed to be the first order kinetics characteristics within 120 min, and the linear equation is 10³ × Y = 0.0497t +6.5992.
