The intraspecific difference of the triose phosphate isomerase (tim) gene from Giardia lamblia.
- Author:
Siqi LU
1
;
Jihong LI
;
Yaping ZHANG
;
Jianfan WEN
;
Fengyun WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Base Sequence; DNA, Protozoan; chemistry; genetics; Giardia lamblia; enzymology; genetics; Molecular Sequence Data; Phylogeny; Polymerase Chain Reaction; Sequence Alignment; Sequence Analysis, DNA; Sequence Homology, Nucleic Acid; Species Specificity; Triose-Phosphate Isomerase; genetics
- From: Chinese Medical Journal 2002;115(5):763-766
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate the intraspecific difference of the triose phosphate isomerase (tim) gene from Giardia lamblia (G. lamblia).
METHODSTotal genomic DNA of G. lamblia was extracted and partial fragments of the triose phosphate isomerase (tim) gene were amplified by polymerase chain reaction (PCR). All nucleotide sequences were analyzed by using a phylogenetic analysis, which was constructed with parsimony and Neighbor-joining (N-J) methods.
RESULTSA total of 124 variable sites (23% of all sequences detected) was defined, most of which were found at the silent sites of codons. Two similar phylogenetic trees were constructed, subdividing 16 Giardia isolates into two groups.
CONCLUSIONThe genetic diversity of G. lamblia appeared to be little affected by factors of both host and geography, while natural-selection played an important role in DNA molecular evolution level of the tim gene. The tim gene may be considered a very useful genetic marker of the population genetic structure of G. lamblia.
