Decreased expression of AQP1 and AQP5 in acute injured lungs in rats.
- Author:
Guangyu JIAO
1
;
Erran LI
;
Runjiang YU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Aquaporin 1; Aquaporin 5; Aquaporins; analysis; Immunohistochemistry; Lipopolysaccharides; toxicity; Lung; metabolism; Male; Membrane Proteins; Microscopy, Immunoelectron; Rats; Rats, Sprague-Dawley; Respiratory Distress Syndrome, Adult; metabolism; pathology
- From: Chinese Medical Journal 2002;115(7):963-967
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo determine if aquaporin1 (AQP1) and aquaporin5 (AQP5) are expressed in the alveolar capillary membrane in rats. Moreover, to investigate the alteration of AQP1 and AQP5 in acute injured lungs.
METHODSThe distribution of AQP1 and AQP5 in alveolar capillary membrane were investigated by immunohistochemistry and immunoelectron microscopy with affinity-purified antibodies to human AQP1 and AQP5. To study the possibility that alveolar capillary membrane AQP1 and AQP5 undergo altered regulation, we established a rat model using alveolar instillation of lipopolysaccharide (LPS).
RESULTSImmunolabelling showed AQP1 was stained primarily in the microvascular endotheli a of normal lungs, while AQP5 was expressed in type I pneumocytes. Immunohisto chemical analysis showed a significant decrease in the expression of AQP1 and AQP5 in injured lungs at 4h-48h after LPS instillation. AQP1 protein was resumed partly at 24h after LPS instillation and steroid administration, whereas AQP5 was unchanged.
CONCLUSIONThe decreased expressions of AQP1 and AQP5 in injured lungs suggest that both of them may play a role in abnormal fluid transportation.
