Effect of emodin on proliferation and differentiation of 3T3-L1 preadipocyte and FAS activity.
- Author:
Chongben ZHANG
1
;
Lu TENG
;
Yan SHI
;
Junhua JIN
;
Youfang XUE
;
Kegang SHANG
;
Jun GU
Author Information
- Publication Type:Journal Article
- MeSH: 3T3 Cells; Adipocytes; drug effects; physiology; Animals; Cell Differentiation; drug effects; Cell Division; drug effects; Emodin; pharmacology; Fatty Acid Synthases; antagonists & inhibitors; Lipid Metabolism; Mice; Stem Cells; drug effects; physiology
- From: Chinese Medical Journal 2002;115(7):1035-1038
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo study the effects of emodin on proliferation and differentiation of 3T3-L1 preadipocyte and the possible mechanism.
METHODSCell proliferation was determined by MTT spectrophotometry, cell differentiation was determined by Oil Red O staining,and fatty acid synthase (FAS) activity was determined by spectrophotometry.
RESULTSEmodin promoted proliferation of 3T3-L1 preadipocyte at low concentration and inhibited the proliferation at high concentration in a dose-related manner. In contrast, it inhibited cell differentiation into adipocyte at low concentration in a dose-related manner. In vitro emodin inhibited the activity of FAS in a dose-related manner.
CONCLUSIONSThe effects of emodin on 3T3-L1 cell's proliferation and differentiation are dose dependent. Emodin inhibits the activity of FAS. Our results suggest that emodin should have a potential to serve as a fat-reducing drug.
