Tissue tropism of the TTV in experimentally infected rhesus monkeys.
- Author:
Hong XIAO
1
;
Kangxian LUO
;
Shouchang YANG
;
Yanjun WANG
;
Weifang LIANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Bone Marrow; virology; DNA Virus Infections; virology; DNA, Viral; analysis; Intestine, Small; virology; Liver; virology; Macaca mulatta; Torque teno virus; genetics; isolation & purification; physiology; Virus Replication
- From: Chinese Medical Journal 2002;115(7):1088-1090
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo determine whether the transfusion-transmitted virus (TTV) is hepatotropic.
METHODSTotal DNA was extracted from various tissues of 5 experimentally infected Rhesus monkeys during the viremic period. A dot hybridization was done with viral double stranded DNA probes or single antisense probes.
RESULTSThe double-stranded probe was hybridized with DNA from the liver, bone marrow, spleen,stomach, small intestine and colon. The single-stranded antisense probe was hybridized with DNA from the liver, small intestine and bone marrow of all 5 monkeys, but not with that from other tissues.
CONCLUSIONSAs the viral genome was of negative polarity, the plus-stranded fragment identified in our study might be a replicative intermediate, and was only demonstrated in the liver, small intestine, and bone marrow by dot blot hybridization with single-stranded antisense probes. It is suggested that the TTV replicates in the liver, bone marrow and small intestine, and TTV might be hepatotropic.
