Extracellular domain of kinase domain region mediated by adeno-associated virus inhibits growth and angiogenesis of bladder cancer in Balb-c mice.
- Author:
Zhichao ZHANG
1
;
Zhiqing ZHANG
;
Gefei ZENG
;
Liguo ZHANG
;
Chunxiao XU
;
Yinglu GUO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cloning, Molecular; Cricetinae; Dependovirus; genetics; Endothelial Growth Factors; metabolism; Female; Genetic Therapy; Intercellular Signaling Peptides and Proteins; metabolism; Lymphokines; metabolism; Mice; Mice, Inbred BALB C; Mice, Nude; Neovascularization, Pathologic; prevention & control; Urinary Bladder Neoplasms; blood supply; therapy; Vascular Endothelial Growth Factor A; Vascular Endothelial Growth Factor Receptor-2; genetics; Vascular Endothelial Growth Factors
- From: Chinese Medical Journal 2002;115(8):1209-1212
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo verify whether the extracellular domain of kinase domain region (KDR) has anti-angiogenesis activity in vivo.
METHODScDNA was cloned into adeno-associated virus (AAV) vector pSNAV and transfected to baby hamster kidney (BHK) cells. Recombinant AAV was obtained from the cell culture supernatant after adding helper virus. Recombinant AAV-infected human bladder cancer EJ cell line (EJ cells) were injected subcutaneously into Balb-c nude mice. Tumor specimens were removed from the mice, paraffin-embedded and sliced, then stained by immunohistochemistry. Microvessel density (MVD) was determined under a microscope.
RESULTSThe tumor volume developed by EJ cells transfected with the extracellular domain of KDR was significantly smaller (1.70 +/- 0.18 cm(3)) compared with that in the control (5.62 +/- 0.67 cm(3)) (P < 0.05), although tumor developed to be detectable on almost the same time (14.7 +/- 2.4 days vs 14.1 +/- 3.2 days). Further, MVD in the experimental group was lower than that in the control (41.3 +/- 4.8 vs 6.2 +/- 2.1, P < 0.05).
CONCLUSIONThe extracellular domain of KDR could be expressed in nude mouse bladder cancer tissue and inhibit tumor angiogenesis.
