Identification of Trichophyton rubrum by PCR fingerprinting.
- Author:
Hongmei ZHU
1
;
Hai WEN
;
Wanqing LIAO
Author Information
- Publication Type:Journal Article
- MeSH: DNA Fingerprinting; methods; Humans; Polymerase Chain Reaction; methods; Trichophyton; genetics; isolation & purification
- From: Chinese Medical Journal 2002;115(8):1218-1220
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo observe the unique DNA profile and the relationship between DNA profile and phenotype of Trichophyton rubrum,and establish an effective molecular method to identify T. rubrum.
METHODSThree primers, including (GACA)(4), (GTG)(5) and M13 core sequence (5'-GAGGGTG-GCGGTTCT-3'), were used to distinguish variations among 20 clinical isolates of T. rubrum and Trichophyton mentagrophytes.
RESULTSDifferent PCR-fingerprinting was seen between T. rubrum and T. mentagrophytes using three different primers. 2 stains of T. rubrum were identified among 6 supposed T. mentagrophytes.
CONCLUSIONST. rubrum can be distinguished using PCR, and (GACA)(4) is the most suitable primer.
