Mcl-1 mediates cytokine deprivation induced apoptosis of human myeloma cell line XG-7.
- Author:
Lun SONG
1
;
Yan LI
;
Yingxun SUN
;
Beifen SHEN
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Humans; Interleukin-6; physiology; Multiple Myeloma; pathology; Myeloid Cell Leukemia Sequence 1 Protein; Neoplasm Proteins; analysis; physiology; Proto-Oncogene Proteins c-bcl-2; analysis; Tumor Cells, Cultured; bcl-X Protein
- From: Chinese Medical Journal 2002;115(8):1241-1243
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate apoptosis in XG-7, a human myeloma cell line, induced by IL-6 deprivation and the function of three anti-apoptotic Bcl-2 proteins (Bcl-2, Bcl-kappa(L), Mcl-1) in the apoptotic process.
METHODSApoptosis in XG-7 myeloma cells induced by IL-6 withdrawal was determined by flow cytometry with propidium iodide (PI) nuclear staining. Expressions of three Bcl-2 proteins in XG-7 cells were monitored by immunoblotting assay.
RESULTSIn the absence of IL-6 for a certain time, a significant percentage of apoptiotic XG-7 cells can be observed, as well as down-regulated expression of one of the three anti-apoptotic proteins (Mcl-1) in XG-7 cells. IL-6 re-stimulation in XG-7 cells following cytokine removal up-regulated the expression of Mcl-1 and inhibited cell apoptosis.
CONCLUSIONMcl-1,instead of Bcl-2 and Bcl-kappa(L), plays an important role in IL-6 deprivation induced apoptosis in XG-7 human myeloma cells.
