Decline in the expression of IL-2 after trauma and changes in the nuclear transcription factors NFAT and AP-1.
- Author:
Yan LUO
1
;
Huaping LIANG
;
Chenxiang HU
;
Xiang XU
;
Zhengguo WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Nucleus; chemistry; DNA; metabolism; DNA-Binding Proteins; metabolism; Electrophoretic Mobility Shift Assay; Female; Interleukin-2; analysis; genetics; Male; Mice; NFATC Transcription Factors; Nuclear Proteins; Proto-Oncogene Proteins c-fos; analysis; Proto-Oncogene Proteins c-jun; analysis; RNA, Messenger; analysis; Transcription Factor AP-1; metabolism; Transcription Factors; metabolism
- From: Chinese Medical Journal 2002;115(9):1348-1351
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate whether the decrease in expression of interleukin-2 (IL-2) after trauma is associated with changes in DNA binding activity of nuclear factor of activated T cells (NFAT) and activator protein-1 (AP-1).
METHODSMice with closed impact injury with fracture in both hind limbs were adopted as the trauma model. Spleen lymphocytes were isolated from traumatized mice and stimulated with Con-A. Culture supernatants were assayed for IL-2 activity, and total RNA was extracted from spleen lymphocytes and assayed for IL-2 mRNA. DNA binding activity of NFAT and AP-1 were measured by electrophoretic mobility shift assay (EMSA). The expression of c-Fos, c-Jun and JunB proteins was determined by the Western blot analysis.
RESULTSDNA binding activity of NFAT and AP-1 gradually decreased to a minimum of 41% and 49%, respectively, of the control on the 4th day after injury, which was closely followed by the decline in IL-2 activity and IL-2 mRNA. A decrease in the expression of c-Fos on the 1st and 4th day after trauma had no significant effect on c-Jun expression; the increase in expression of JunB was only on the 1st day after injury.
CONCLUSIONDecreased IL-2 expression is, at least in part, due to a decline in the activation of NFAT and AP-1 in traumatized mice. The decline in DNA binding activity of NFAT and AP-1 is partly due to a trauma-induced block in the expression of c-Fos.