Hypoxia enhances the proliferation of skeletal myoblasts and possible mechanism.
- Author:
Fan WANG
1
;
Ling-ling ZHU
;
Xiao-ping CHEN
;
Guo-shu LIU
;
Ming FAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Hypoxia; Cell Proliferation; Cells, Cultured; Hypoxia-Inducible Factor 1, alpha Subunit; genetics; metabolism; Male; Myoblasts, Skeletal; cytology; metabolism; Oxygen; metabolism; RNA, Messenger; genetics; metabolism; Rats; Rats, Wistar
- From: Chinese Journal of Applied Physiology 2010;26(2):133-136
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the role of hypoxia in skeletal myoblasts proliferation and related mechanism.
METHODSThe numbers and proliferous indexes of skeletal myoblasts were detected by flow cytometer under 20%, 3%, and 10% oxygen concentration. Hypoxia inducing factor 1alpha (HIF-1alpha) mRNA expression was determined by reverse transcriptase-polymerase chain reaction (RT-PCR). HIF-1alpha proteinum in endochylema and intranucelus were respectively detected by Western blot.
RESULTSThe numbers and proliferous indexes were higher (P < 0.05) in hypoxia group than those of control group. The expression HIF-1alpha mRNA had no difference in hypoxia and in normal groups. The level of HIF-1alpha proteinum in endochylema under normoxia was more than that in intranucelus and it was opposite under hypoxia.
CONCLUSIONHypoxia can promote the proliferation of skeletal myoblasts. The possible mechanism of hypoxia promoting the proliferation of skeletal myoblasts might be that low oxygen concentration regulates HIF-la nuclear translocation.
