Effects of Yikunning on the apoptotic rate and expression of caspase-3 in rat ovaries during perimenopausal period.
- Author:
Qing-Zhi CUI
1
;
Yan-Jun TIAN
;
Hai-Xia WEN
;
Guo-Dong ZHANG
;
Rui-Na HAN
;
Guang-Mei ZHANG
;
Guo-Yi LI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; drug effects; Caspase 3; metabolism; Drugs, Chinese Herbal; pharmacology; Female; Ovary; cytology; metabolism; Perimenopause; Rats; Rats, Wistar
- From: Chinese Journal of Applied Physiology 2010;26(3):318-321
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of Yikunning (compound of Chinese traditional Medicine, YKN) on the apoptotic rate and expression of caspase-3 in rat ovaries during perimenopausal period.
METHODSThirty female Wistar rats during perimenopausal period were selected by unforced aging. Then the rats were divided into 3 groups randomly: YKN group, livial control group and aged control group. Ten young female rats were selected as young control group. Intragastric administrations were conducted for 4 weeks once daily continuously. The apoptotic rate in rat ovaries were detected by TUNEL. The expression of caspase-3 mRNA and protein in rat ovaries were detected by RT-PCR and Western blot, respectively.
RESULTSThe apoptotic rate in rat ovaries in YKN group was lower than that in aged control group, which showed difference between them (P < 0.01). The levels of caspase-3 mRNA and protein in rat ovaries in YKN group were lower than those in aged control group, which showed differences among them (P < 0.01).
CONCLUSIONYKN can decrease the apoptotic rate and down-regulate the expression of caspase-3 mRNA and protein in rat ovaries of during perimenopausal period. It may be one of the molecular mechanisms of YKN postponed the ovarian failure and cured perimenopausal syndrome.
