Effect of cisplatin on apoptosis of spiral ganglion cell and expression of caspase-3 in mouse cochlea.
- Author:
Wei-Xia SUN
1
;
Ai-Mei WANG
;
Cui-Fen BAO
;
Li-Jie HUI
;
Zhi-Jie CHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antineoplastic Agents; pharmacology; Apoptosis; drug effects; Caspase 3; metabolism; Cisplatin; pharmacology; Cochlea; cytology; drug effects; metabolism; Male; Mice; Mice, Inbred Strains; Spiral Ganglion; cytology; drug effects; metabolism
- From: Chinese Journal of Applied Physiology 2010;26(3):324-328
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a mice model of cisplatin-induced ototoxicity, and to investigate the effect of cisplatin on apoptosis of spiral ganglion cell and expression of caspase-3 in mouse cochlea.
METHODSTerminal deoxynucleotidyl transferase-mediated nick end labeling method (TUNEL) was used to monitor the apoptosis of spiral ganglion cell. Envision method of immunohistochemistry was applied to detect the expression of caspase-3 in cochlea. Auditory brainstem response (ABR) was measured to observe the change of hearing.
RESULTSThe weight and hearing of mice in different dose of cisplatin groups were declined significantly as compared with those of control group (P < 0.05, P < 0.01), and the TUNEL positive cell number and expression of caspase-3 were greater remarkably with the more cisplatin injected.
CONCLUSIONA mouse model of cisplatin-induced ototoxicity can be established. Cisplatin can lead to the apoptosis of spiral ganglion cells, and caspase-3 has participated in this apoptosis process, which approves further that apoptosis might be one of the mechanisms of cisplatin ototoxicity.
