Inhalation of glucocorticosteroid in the rat model of bleomycin-induced pulmonary fibrosis and its impact on the expression of basic fibroblast growth factor.
- Author:
Jian-Qin MU
1
;
Guo-Qiang LI
;
Lu-Qing WEI
Author Information
- Publication Type:Journal Article
- MeSH: Administration, Inhalation; Animals; Fibroblast Growth Factor 2; metabolism; Glucocorticoids; administration & dosage; therapeutic use; Male; Pulmonary Fibrosis; drug therapy; metabolism; pathology; Rats; Rats, Wistar
- From: Chinese Journal of Applied Physiology 2010;26(3):330-333
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of inhalation of glucocorticoid on bleomycin-induced pulmonary fibrosis in rats and its possible mechanism.
METHODSPulmonary fibrosis was induced in Wistar rats by intratracheal instillation of bleomycin (group M, group B, group D). Group B inhaled glucocorticoid daily from the next day of received bleomycin. Group D intraperitoneal injection glucocorticoid daily from the next day of received bleomycin. Normal controls received normal saline both intratracheally. Five rats in each group were killed at 1, 4 week after intratracheal instillation. Histological changes of the lungs were evaluated by HE, Masson trichrome stain. Lung expression of bFGF proteins was assessed by immunohistochemistry and the level of bFGF protein in serum and BALF was further measured by ELISA.
RESULTSPulmonary fibrosis of group M was higher than that of group C, pulmonary fibrosis of group B, D was lower than that of group M at 1, 4 week. bFGF in group M was higher than that in group C, bFGF in group B, D was lower than that in group M in lung, serum and BALF on 1, 4 week.
CONCLUSIONInhalation of glucocorticosteroid alleviates bleomycin-induced pulmonary fibrosis in rats. The mechanism may be related to the changes that bFGF is degrade or prevent it step up.
