Effect of progesterone on MMP-3 expression in neonatal rat brain after hypoxic-ischemia.
- Author:
Chun-Yang XU
1
;
Shuang LI
;
Xin-Qiang LI
;
Dong-Liang LI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Animals, Newborn; Blood-Brain Barrier; physiopathology; Hypoxia-Ischemia, Brain; metabolism; pathology; physiopathology; Matrix Metalloproteinase 3; metabolism; Progesterone; pharmacology; Rats; Rats, Sprague-Dawley
- From: Chinese Journal of Applied Physiology 2010;26(3):370-373
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of progesterone on matrix metalloproteinase-3 (MMP-3) expression in neonatal rat brain after hypoxic-ischemia.
METHODSFollowed the hypoxic-ischemia of neonatal rat brain, Evans blue (EB) staining and transmission electron microscopy were used to detect the blood-brain barrier pathological changes on permeability. MMP-3 protein expression in cerebral cortex was measured with Western blot.
RESULTSTransmission electron microscopy results showed that the blood brain barrier in hypoxic-ischemic group changed significantly compare to progesterone group. EB staining results suggested that the blood-brain barrier permeability of hypoxic-ischemic group was significantly increased compared to sham-operated group (P < 0.01). The blood-brain barrier permeability in progesterone group was also decreased in comparison to that of hypoxic-ischemic group (P < 0.05). Western blot image analysis results indicated that MMP-3 protein expression in the hypoxic-ischemic group increased significantly than that in sham-operated group (P < 0.01), and the progesterone group was decreased significantly than that in hypoxic-ischemic group (P < 0.05).
CONCLUSIONProgesterone may reduce the blood-brain barrier damage by reducing MMP-3 expression. This might be one of the protective mechanisms in the hypoxic-ischemic brain injury.
