Effect of fractalkine on proliferation of pulmonary artery smooth muscle cells.
- Author:
Xiao-Ju CHEN
1
;
De-Yun CHENG
;
Qiao-Li SU
;
Li-Li FAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Proliferation; drug effects; Cells, Cultured; Chemokine CX3CL1; pharmacology; Male; Muscle, Smooth, Vascular; cytology; Myocytes, Smooth Muscle; cytology; drug effects; Pulmonary Artery; cytology; Rats; Rats, Sprague-Dawley
- From: Chinese Journal of Applied Physiology 2009;25(4):445-448
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo investigate the effect of fractalkine on cell proliferation of cultured rat pulmonary artery smooth muscle cells (PASMCs) in vitro.
METHODSRat PASMCs were cultured in vitro, and treated with different concentrations (10(-10), 10(-9), 10(-8) mol/L) of fractalkine for 12 h, 24 h and 48 h. The cell proliferation was quantified by MTT assay. The cell cycle of PASMCs was measured by flow cytometric(FCM) analysis.
RESULTSMTT assay showed that fractalkine promoted significantly the proliferation of PASMCs, and the effect was concentration-dependent. FCM analysis indicated that fractalkine increased the percentage of S phase and proliferative index (PI). The percentage of S phase and PI of PASMCs were increased after treated with fractalkine for 12 hours, which reached a maximal level at 24 hours.
CONCLUSIONFractalkine promotes rat PASMCs proliferation in a concentration-dependent manner.
