Study on drug-resistance of Mycobacterium tuberculosis isolated from coal workers with silico-tuberculosis.
- Author:
Ping JIANG
1
;
Hongmin LI
;
Dongjin CHEN
;
Wei WANG
;
Bai FENG
;
Zhongyuan WANG
;
Guoyang WANG
;
Sumei LI
;
Huixin HAN
Author Information
- Publication Type:Journal Article
- MeSH: Coal; Drug Resistance, Bacterial; Humans; Mutation; Mycobacterium tuberculosis; drug effects; genetics; Polymerase Chain Reaction; Polymorphism, Single-Stranded Conformational; Silicotuberculosis; microbiology; Sputum; microbiology
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2002;20(2):100-102
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the gene mutation and streptomycin, isoniazid or rifampicin resistance of Mycobacterium isolated from silico-tuberculosis patient's sputum so as to find a more effective therapy for this disease.
METHODSMycobacteria tuberculosis were separated from 96 coal worker with silico-tuberculosis firstly. Then rpsL, KatG and rpoB fragments of genome were copied with PCR and compared their SSCP profiles with standard strains.
RESULTS67 strains of streptomycin, isoniazid or rifampicin resistant Mycobacteria tuberculosis were found in routine drug resistance test, with the percentages of 80.5% (54/67), 58.2% (39/67) respectively. PCR-SSCP showed that out of 67 drug-resistant strains, 66(98.5%) of rpsL, 47(70.1%) of rpoB and 42(62.7%) of KatG appeared abnormal.
CONCLUSIONMost of the resistant strains appeared gene mutation. The mution rates were higher than the results from routine drug resistance test.
