Study on making pneumoconiosis model and method of lung lavage in rabbit.
- Author:
Dong XU
1
;
Yiming WU
;
Peng WANG
;
Yubao XU
;
Aimin MENG
;
Hong LI
;
Zhangxian LIU
;
Ran ZHU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Bronchoalveolar Lavage; methods; Coal; Disease Models, Animal; Dust; Lung; pathology; Pneumoconiosis; etiology; Rabbits; Silicon Dioxide
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2002;20(2):131-133
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo provide an intact rabbit pneumoconiosis model and a method of lung lavage for studying the pathogenesis and treatment of pneumoconiosis.
METHODSThe dust particles(< or = 5 microns diameter) were poured into the trachea of rabbit by trachea spile. The lung lavage of rabbit was carried out by the improved trachea catheter method.
RESULTSThe rabbit lungs exposed to coal dust showed many black dust spots and there were proliferation of fibroblasts. The rabbit lungs exposed to silica dust showed nodules and several fusing nodules. The X-ray film showed that there were small irregular shadows, or fusing together into flake-like or round shadows in both lungs. The recovery rate of the lung lavage was 89%-94%.
CONCLUSIONThe model made by trachea spile and the method of lung lavage by the improved trachea catheter were simple, safe and without being injured.
