The study of DNA damage of peripheral lymphocytes in the nurses occupationally exposed to anticancer drugs.
- Author:
Dongping YANG
1
;
Shijie XU
;
Jianxin WANG
Author Information
- Publication Type:Journal Article
- MeSH: Antineoplastic Agents; adverse effects; DNA Damage; Humans; Lymphocytes; drug effects; Nurses; Occupational Exposure; adverse effects
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2002;20(3):197-199
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo detect the DNA damage of the nurses occupationally exposed to anticancer drugs and to assess the exposure level using Comet assay.
METHODSSixteen nurses occupationally exposed to anticancer drugs were selected as exposure group, the average exposure period was 5.6 years, and the average exposure dose was to prepare 7.8 portions of anticancer drugs daily. Meanwhile, sixteen nurse students were selected as control group. The DNA migration of the peripheral lymphocytes of both groups was detected using comet assay.
RESULTSThe comet length was 46.27 microns in exposure group, which was significantly higher than that of control group (26.78 microns, P < 0.01). Also the percentage of long tailed nucleus (LTN) of exposure group was 64.83%, which was significantly higher than that of control group (4.87%, P < 0.01).
CONCLUSIONSThere was DNA damage in the nurses occupationally exposed to antineoplastic drugs.
