The effects of electromagnetic pulse on fluidity and lipid peroxidation of mitochondrial membrane.
- Author:
Changzhen WANG
1
;
Jianbo CONG
;
Hong XIAN
;
Xiaozhe CAO
;
Cunpu SUN
;
Ke WU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Electromagnetic Phenomena; Lipid Peroxidation; radiation effects; Membrane Fluidity; radiation effects; Mitochondria, Liver; metabolism; radiation effects; Mitochondrial Membranes; metabolism; radiation effects; Rats
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2002;20(4):266-268
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects of intense electromagnetic pulse(EMP) on the biological effects of mitochondrial membrane.
METHODRat liver mitochondrial suspension was exposed to EMP at 60 kV/m level. The changes of membrane lipid fluidity and membrane protein mobility were detected by ESR and spin label technique. Malondialdehyde(MDA) was detected by spectrophotometer.
RESULTSThe mobility of membrane protein decreased significantly(P < 0.05). Correlation time (tau c) of control group was (0.501 +/- 0.077) x 10(-9)s, and tau c of EMP group was (0.594 +/- 0.049) x 10(-9)s, indicating that the mobility of protein was restricted. The fluidity of mitochondrial membrane increased significantly(P < 0.05) at the same time. Order parameter(S) of mitochondrial membrane lipid in control group was 0.63 +/- 0.01, while S of EMP group was 0.61 +/- 0.01(P < 0.05). MDA decreased significantly.
CONCLUSIONThe mobility and lipid peroxidation of mitochondrial membrane may be disturbed after EMP exposure.
