Effect of low-intensity microwave of on mitomycin C-induced genotoxicity in vitro.
- Author:
Meibian ZHANG
1
;
Deqiang LU
;
Jiliang HE
;
Lifen JIN
Author Information
- Publication Type:Journal Article
- MeSH: Chromosome Aberrations; Comet Assay; DNA Breaks, Single-Stranded; Female; Humans; Lymphocytes; drug effects; radiation effects; ultrastructure; Male; Micronuclei, Chromosome-Defective; Microwaves; adverse effects; Mitomycin; toxicity
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2002;20(4):273-276
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study that low-intensity microwave whether or not enhances the genotoxic effects of mitomycin C(MMC) on human lymphocytes.
METHODSSingle strand DNA breaks and chromosomal aberrations were measured by comet assay and cytokinesis-blocked micronucleus(CBMN) test in vitro when human lymphocytes were exposed to 2,450-MHz microwave (5.0 mW/cm2) alone and in combination with mitomycin C.
RESULTSIn the comet assay, the average comet lengths of microwave group[(29.1 +/- 8.1) micron in male and (25.9 +/- 7.5) micron in female] were not significantly different from those of control groups [(26.3 +/- 6.6) and (24.1 +/- 4.3) micron respectively] (P > 0.05). The average comet lengths of MMC group(0.0125, 0.0250, 0.0500, 0.1000 microgram/ml) were significantly longer than those of control groups (P < 0.01) and were increased with the dose of MMC. The average comet lengths of microwave combined with MMC (MW + MMC) also were increased with the doses of MMC and were significantly longer than those of control groups (P < 0.01). When MMC was > or = 0.0250 microgram/ml, microwave and MMC synergistically increased the single strand DNA breaks. In the micronucleus test, the average micronucleus rates of microwave groups were not higher than those of control groups (P > 0.05). The average micronucleus rates of MMC groups and MW + MMC groups were significantly higher than those of control groups (P < 0.01) when MMC was > or = 0.0500 microgram/ml. The average micronucleus rates of MW + MMC groups seemed higher than those of corresponding MMC groups, however the difference was not significant (P > 0.05).
CONCLUSIONLow-intensity(2,450-MHz) microwave did not induce DNA and chromosome damages on human lymphocytes, but enhanced the effects of DNA breaks induced by MMC.