Difference in lymphocyte heat shock protein 71 (HSP71) expression in soldiers of armed police forces from different climatic zones.
- Author:
Huasong ZENG
1
;
Chengfeng XIAO
;
Xiujing JING
;
Miao YANG
;
Lei KE
;
Qian XU
;
Hao TAN
;
Fangze LI
;
Tangchun WU
Author Information
- Publication Type:Journal Article
- MeSH: Climate; Comet Assay; DNA Damage; Flow Cytometry; Heat Stress Disorders; metabolism; Heat-Shock Proteins; biosynthesis; Humans; Lymphocytes; metabolism; Military Personnel; Police
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2002;20(4):282-284
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo analyze the difference between basal and heat-inducible levels of lymphocyte heat shock protein 71 (HSP71) expression in soldiers from Beijing, Zhengzhou and Guangzhou.
METHODSFlow cytometry and Comet assay were used to detect the level of HSP71 and DNA damage respectively.
RESULTSComet assay showed that there was no significant DNA damage before and after heat stress at 41 degrees C for 1 h, and also no difference found among the 3 climatic zones(P > 0.05). HSP71 of all soldiers in the 3 zones elevated after stress (P < 0.05). The basal and heat-inducible levels of HSP71 in Beijing soldiers(845.87 +/- 135.60 and 1254. 47 +/- 239.05 mean fluorescence intensity respectively) were higher than those in Guangzhou soldiers(702.73 +/- 184.70 and 861.72 +/- 225.12 mean fluorescence intensity respectively) (P < 0.05).
CONCLUSIONThe differences of lymphocyte HSP71 expression before and after heat stress among the soldiers from Beijing, Zhengzhou and Guangzhou suggest that basal and heat-inducible levels of lymphocyte HSP71 expression may be considered as a valuable index to evaluate heat tolerance of soldiers in different climatic zones.
