Study on the difference in DNA damage of human lymphocyte caused by arsenicals.
- Author:
Xin LI
1
;
Xiance SUN
;
Guifan SUN
;
Yaping JIN
;
Bing LI
;
Xiaoying GUO
;
Shan LIU
Author Information
- Publication Type:Journal Article
- MeSH: Arsenates; toxicity; Arsenites; toxicity; Comet Assay; DNA Damage; Dose-Response Relationship, Drug; Humans; Lymphocytes; drug effects; ultrastructure
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2002;20(5):327-330
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore whether there is difference in arsenicals-induced DNA damage of human lymphocyte.
METHODSLymphocyte were sterilely collected from healthy donor and exposed to sodium arsenite (AsIII), sodium arsenate(AsV) and methyl sodium arsenate(MAsv) at 1,5,10,20 and 50 mumol/L. After incubation of 24 hours, cells were collected by centrifugation and DNA damage was detected by single cell gel electrophoresis (SCGE).
RESULTSThe comet frequency distribution of all groups except 1 mumol/L group of MAsV were significantly different from that of control. The comet length of all groups except 1 mumol/L group of AsV and 1.5 mumol/L groups of MAsV were significantly higher than that of control. There were correlations between the doses of arsenicals and the ratios of comet cell or length of comet(rAsIII = 0.8134, rAsV = 0.8734, rMAsV = 0.8994).
CONCLUSIONDNA damage in human lymphocyte were induced by all the three arsenicals. A dose-effect relationship was observed between exposure doses of the same arsenical and DNA damage. With different arsenicals but the same exposure dose, the DNA damage level was as follow: AsIII > AsV > MAsV.
