Relation of genetic polymorphism of NQO1 and GSTT1 with risks of chronic benzene poisoning.
- Author:
Junxiang WAN
1
;
Jinxiu SHI
;
Jiru GUAN
;
Rong YE
;
Xiaoling GAO
;
Weiwei LIU
;
Lijian HUI
;
Duozhi CAO
;
Xipeng JIN
;
Gengxi HU
;
Zhaolin XIA
Author Information
- Publication Type:Journal Article
- MeSH: Benzene; poisoning; Case-Control Studies; Ethanol; adverse effects; Genotype; Glutathione Transferase; genetics; Humans; NAD(P)H Dehydrogenase (Quinone); genetics; Occupational Diseases; genetics; Occupational Exposure; Polymorphism, Single Nucleotide; Smoking; adverse effects
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2002;20(5):340-343
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the relation between genetic polymorphisms of NQO1, GSTT1 and risks of chronic benzene poisoning (BP).
METHODSA case-control study was conducted. 152 BP patients and 152 workers occupationally exposed to benzene without poisoning manifestations were investigated. Polymerase chain reaction (PCR), denaturing high-performance liquid chromatography(DHPLC) and sequencing were used to detect the single nucleotide polymorphisms(SNPs) of the promoter and complete coding-region of NQO1 gene. Multiple PCR was used to detect GSTT1 genotype.
RESULTSIn smoking population, there was 7.73-fold (95% CI: 1.71-34.97, P = 0.010) of risk in BP subjects carrying NQO1c. 609 T/T genotype, compared with those carrying C/C and C/T. genotype. In drinking population, the individuals carrying the 6th extron of NQO1c. 609 T/T homozygote genotype had a 11.00-fold(95% CI: 1.89-63.83, P = 0.005) risk of BP compared to those with NQO1c. 609 C/T and C/C genotypes.
CONCLUSIONThe subjects carrying NQO1c. 609 T/T genotype and together with the habit of smoking or drinking may be more susceptible to BP.
