Study on three kinds of gasoline oxygenates-induced DNA damage in mice fibroblasts.
- Author:
Chonglin SONG
1
;
Zhifu ZHANG
;
Xue CHEN
;
Yanfeng ZHANG
;
Chunhua WANG
;
Keming LIU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Comet Assay; DNA Damage; Dose-Response Relationship, Drug; Ethanol; toxicity; Fibroblasts; drug effects; Formates; toxicity; Methyl Ethers; toxicity; Mice
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2002;20(5):362-364
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study DNA damage of three kinds of gasoline oxygenates.
METHODSingle cell gel electrophoresis assay(Comet assay) was used to detect the damage effects of three gasoline oxygenates[methyl tertiary butyl ether(MTBE), ethanol anhydrous(EA) and dimethyl carbonate(DMC)] on DNA in L-929 mice fibroblasts.
RESULTSIn certain concentation(37.500-150.000 mg/ml), MTBE could directly cause DNA damage of L-929 mice fibroblasts. There was obvious dose-effect relationship, i.e. when the concentration of MTBE was increased from 9.375 to 150.000 mg/ml, the comet rate also increased from 4% to 85%, and the length of comet tail changed correspondingly. The results of EA and DMC were negative.
CONCLUSIONUnder the condition of this experiment(150.000 mg/ml), MTBE could directly cause DNA damage while the effect of EA and DMC on DNA damage was not found.
