An immunohistochemical study of the effects of excessive fluoride on type I collagen in rat developmental dentine.
- Author:
Yu-qing HAO
1
;
Gou-quan WANG
;
Zhong-ying NIU
;
Xue-dong ZHOU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Animals, Newborn; Collagen Type I; analysis; Dentin; chemistry; drug effects; growth & development; Fluorides; toxicity; Immunohistochemistry; Rats; Rats, Sprague-Dawley
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2003;21(6):429-431
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects of excessive fluoride on type I collagen in rat developmental dentine.
METHODSEighty SD rats, 5 days old, were divided into experimental and control groups, 40 in each group. The experimental group received subcutaneous injection of 0.2% NaF every 4 days (the dose was 2 mg NaF per kg body wt). The same volume of 0.9% NaCl was used in the control. Twenty rats in each group were killed 4 days after the second and the seventh injection respectively. The expression of type I collagen was assayed with immunohistochemical technique.
RESULTS4 out of 20 rats after two injections showed abnormal distribution of type I collagen (dense stain of collagen in the odontoblast, aggregation of collagen in the dentine and disordered arrangement of collagen in the predentine; All 20 rats after seven injections showed abnormal distribution of type I collagen.
CONCLUSIONExcessive fluoride may affect the metabolism of type I collagen in rat developmental dentine.
