Detection of DNA damage induced by carbon disulfide in mice sperm with single-cell gel electrophoresis assay.
- Author:
Guo-hui TANG
1
;
Deng-feng XUAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Carbon Disulfide; toxicity; Comet Assay; DNA Damage; Male; Mice; Spermatozoa; chemistry; drug effects
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2003;21(6):440-443
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the genotoxicity of carbon disulfide by detecting DNA damage in mice sperm with single-cell gel electrophoresis assay (SCGE).
METHODSSCGE was used to detect sperm DNA damage. The index of DNA damage, tail length and tail moment were used to evaluate the extent of DNA damage.
RESULTSIn three dosage groups, the rate of DNA damage (67.14%, 84.29% and 91.00%, respectively), index of DNA damage intensity (507, 656 and 745, respectively), tail length (5.87, 8.81 and 13.49 microm, respectively) and tail moment (1.30, 1.63, 2.66 microm, respectively) were significantly increased, while the percentage of head of the comet was significantly decreased (84.55%, 73.84% and 55.71%, respectively). A significant changes were clearly observed in all dosage groups compared to those of the control group (P<0.05).
CONCLUSIONSCGE which is a quick and sensitive method to detect DNA damage induced by CS2 may be used to monitor carcinogen and mutagen.
