Effects of extremely low frequency electromagnetic fields on the level of c-fos mRNA in brain and liver of mouse.
- Author:
Yun LIU
1
;
Yun-mei YU
;
En-qi WENG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Brain; metabolism; radiation effects; Dose-Response Relationship, Radiation; Electromagnetic Fields; Gene Expression Regulation; radiation effects; Genes, fos; genetics; Liver; metabolism; radiation effects; Male; Mice; RNA, Messenger; genetics; metabolism; Reverse Transcriptase Polymerase Chain Reaction; Time Factors
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2003;21(5):335-338
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects of extremely low frequency electromagnetic fields (ELF EMFs) on c-fos gene expression in mouse brain and liver tissues.
METHODSMice were exposed to 50 Hz sinusoidal 0.2 mT or 6.0 mT electromagnetic field for 2 weeks or 4 weeks. Competitive RT-PCR method was used to measure c-fos mRNA level.
RESULTSAfter exposure to 0.2 mT or 6.0 mT field for 2 weeks, c-fos mRNA levels in brain tissue [(0.0178 +/- 0.0076) amol/120 ng cDNA and (0.0092 +/- 0.0042) amol/120 ng cDNA respectively] were higher than that of control level [(0.0012 +/- 0.0005) amol/120 ng cDNA] (P < 0.05). In liver tissue c-fos mRNA levels [(0.0117 +/- 0.0055) amol/120 ng cDNA and (0.0148 +/- 0.0162) amol/120 ng cDNA respectively] were also higher than that of control level [(0.0005 +/- 0.0005) amol/120 ng cDNA] (P < 0.05). After exposure to 0.2 mT or 6.0 mT field for 4 weeks, c-fos mRNA levels in brain tissue [(0.0100 +/- 0.0054) amol/120 ng cDNA and (0.0198 +/- 0.0079) amol/120 ng cDNA respectively] were higher than that of control level [(0.0015 +/- 0.0008) amol/120 ng cDNA] (P < 0.05). In liver tissue the exposure induced much higher expression level [(0.0173 +/- 0.0122) amol/120 ng cDNA and (0.0133 +/- 0.0090) amol/120 ng cDNA respectively] while no expression was found in the control.
CONCLUSIONExposure to 50 Hz electromagnetic fields may induce up-regulation of c-fos transcription in mouse brain and liver tissue.
