Effects of extremely low frequency electromagnetic fields on apoptosis and cell cycle of mouse brain and liver cells.
- Author:
Yun LIU
1
;
Rong HONG
;
Yun-mei YU
;
En-qi WENG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; radiation effects; Brain; cytology; radiation effects; Cell Cycle; radiation effects; Electromagnetic Fields; Flow Cytometry; In Situ Nick-End Labeling; Liver; cytology; radiation effects; Male; Mice
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2003;21(5):339-341
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects of extremely low frequency electromagnetic fields (ELF EMFs) on apoptosis and cell cycle of mouse brain and liver cells.
METHODSMice were exposed to 50 Hz, 0.2 mT or 6.0 mT electromagnetic fields for 2 weeks. TUNEL and flow cytometric methods were used to analyze apoptosis and cell cycle of brain and liver cells.
RESULTSAfter exposure to 0.2 mT and 6.0 mT ELF EMFs for 2 weeks, apoptosis rates of brain cells [(5.60 +/- 1.47)% and (4.73 +/- 0.48)% respectively] were higher than that of control [(2.90 +/- 0.75)%], and apoptosis rates of liver cells [(4.19 +/- 2.08)% and (3.38 +/- 0.65)% respectively] were higher than that of control [(1.84 +/- 0.76)%]. G0/G1 cell percentage of brain cells [(80.21 +/- 1.68)% and (79.54 +/- 0.56)% respectively] were higher than that of control [(76.85 +/- 0.83)%], and those of liver cells [(79.42 +/- 1.80)% and (80.47 +/- 1.79)% respectively] were higher than that of control [(73.36 +/- 3.10)%]. The above differences were all statistically significant as P < 0.05. At the same time S and G2 + M cell percentage of brain and liver cells were significantly decreased.
CONCLUSIONExposure to 50 Hz EMFs may alter cell cycle and induce apoptosis of mouse brain and liver cells.
