The expression of cytochrome P450 1A1 of endothelial cells induced by benzo(a)pyrene.
- Author:
Jin-bo YANG
1
;
Chang-zheng JIANG
;
Lei KE
;
Qian XU
;
Miao YANG
;
Han-zhen HE
;
Bing-chang HU
;
Tang-chun WU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Benzo(a)pyrene; toxicity; Blotting, Western; Cells, Cultured; Cytochrome P-450 CYP1A1; analysis; Dose-Response Relationship, Drug; Endothelium, Vascular; cytology; drug effects; enzymology; Immunohistochemistry; Swine
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2003;21(5):362-364
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effect of benzo(a)pyrene (BaP) on the expression and the activities of cytochrome P450 1A1 (CYP1A1) of porcine aortic endothelial cells.
METHODSPorcine aortic endothelial cells were cultured in vitro, and treated with different concentrations of BaP (0, 0.5, 1.0, 5.0, 10.0 micro mol/L) for 24 hours, CYP1A1 expression was determined by Western blot and immunohistochemistry. At the same time, the ethoxyresorufin-o-deethylase (EROD) activities were measured by spectrofluorometer.
RESULTSBy Western blot, the expression of CYP1A1 of control cells was not found, but the expression of CYP1A1 of cells treated with BaP was found; By immunohistochemistry, only part of endothelial cells treated with BaP had positive expression of CYP1A1. The peak activities of EROD induced by BaP was at the concentration of 0.5 - 1.0 micro mol/L.
CONCLUSIONBaP could induce part of endothelial cells to synthesize CYP1A1. BaP of 0.5 - 1.0 micro mol/L could induce peak activities of EROD.
