Immune efficacy of rabies virus glycoprotein expressed by baculovirus vector.
- Author:
Qi CHEN
1
;
Shou-Feng ZHANG
;
Ye LIU
;
Yun-Hong FU
;
Cheng-Long SUN
;
Yang YANG
;
Ting GONG
;
Fei-Fei SONG
;
Rong-Liang HU
Author Information
1. Military Veterinary Research Institute, Academy of Military Medical Sciences, Key Laboratory of Jilin Province for Zoonosis Control and Prevention, Changchun 130122, China. chenqi19880804@163.com
- Publication Type:Journal Article
- MeSH:
Animals;
Antibodies, Viral;
immunology;
Baculoviridae;
genetics;
metabolism;
Gene Expression;
Genetic Vectors;
genetics;
metabolism;
Glycoproteins;
administration & dosage;
genetics;
immunology;
Humans;
Mice;
Rabies;
immunology;
prevention & control;
virology;
Rabies Vaccines;
administration & dosage;
genetics;
immunology;
Rabies virus;
genetics;
immunology;
Viral Proteins;
administration & dosage;
genetics;
immunology
- From:
Chinese Journal of Virology
2012;28(5):501-505
- CountryChina
- Language:Chinese
-
Abstract:
To construct a recombinant baculovirus expressing glycoprotein (GP) of RV SRV9 strain and test the immunological efficacy in mice, open reading frame of rabies virus GP gene of SRV9 strain was cloned into the shuttle vector Bacmid to construct the recombinant shuttle plasmid Bacmid-G and transfection was performed into S f9 cells with the recombinant shuttle plasmid. CPE appeared in cell cultures was identified by electronmicroscopy. Western-blot, IFA and immunity tests in mice were performed to identify the immunoreactivity and immunogenicity of the expression products. Our results showed a recombinant baculovirus expressing GP protein of rabies virus SRV9 was obtained. The expression products possessed a favorable immunogenicity and fall immunized mice could develop 100% protective level of anti-rabies neutralizing antibody. In conclusion, The SRV9 glycoprotein expressed by the recombinant baculovirus in this study had good immunogenicity and could induce anti-rabies neutralizing antibody, which laid the foundation of further development of rabies subunit vaccine.
