Characterization of S gene of a strain of hantavirus isolated from Apodemus peninsulae in Heilongjiang Province.
- Author:
Lu-Fei CHEN
1
;
Shu-Hong CHEN
;
Kai-Li WANG
;
Jing ZHANG
;
Ji-Hong LI
Author Information
1. Institute for Viral Disease Control and Prevention, Heilongjiang Provincial Center for Disease Prevention and Control, Harbin 150030, China. hljlfc@163.com
- Publication Type:Journal Article
- MeSH:
Animals;
China;
Hantavirus;
chemistry;
classification;
genetics;
isolation & purification;
Hantavirus Infections;
veterinary;
virology;
Humans;
Molecular Sequence Data;
Murinae;
virology;
Phylogeny;
Rodent Diseases;
virology;
Sequence Homology, Amino Acid;
Viral Envelope Proteins;
chemistry;
genetics
- From:
Chinese Journal of Virology
2012;28(5):517-521
- CountryChina
- Language:Chinese
-
Abstract:
In order to study the molecular characterization of the hantavirus isolated from Apodemus peninsulae in Heilongjiang Province, the S gene of a new strain NA33 was amplified, sequenced and analyzed. The results showed that the complete nucleotide sequence of the S gene of NA33 strain was composed of 1 693 nucleotides with TA-rich. The S gene contained one ORF, starting at position 37 and ending at position 1 326, encoding the N protein of 429 amino acid residues, and in line with HTN-based coding. Sequence comparison of the S genes between NA33 and reference hantavirus strains showed that NA33 was more homologous to Amur-like viruses than to the Hantaan (HTN) viruses or the other hantaviruses. Phylogenetic analysis of the amino acid sequence of N proteins showed that NA33 was clustered into the group of Amur-like viruses and was more similar to Far East Russia and Jilin strains isolated from Apodemus peninsulae. The phylogenetic tree indicated a certain degree of host-dependent characteristics and geographical aggregation characteristics of hantanviruses. Furthermore, the amino acid sequence of N protein of NA33 had the conserved amino acid sites of Amur-like viruses. In conclusion, Apodemus peninsulae carried Amur-like viruses in Heilongjiang province and was an important infectious source of hemorrhagic fever with renal syndrome (HFRS).
