Inhibition of virus infection in coxsackievirus-induced myocarditis model by RNA interference.
- Author:
Hai-Lan YAO
1
;
Feng HE
;
Zong-Hui XIAO
;
Zhe-Wei LIU
Author Information
1. Capital Institute of Paediatrics, Beijing 100020, China. helen919@163.com
- Publication Type:Journal Article
- MeSH:
Animals;
Coxsackievirus Infections;
drug therapy;
virology;
Down-Regulation;
Enterovirus B, Human;
genetics;
physiology;
Humans;
Male;
Mice;
Mice, Inbred BALB C;
Myocarditis;
drug therapy;
virology;
RNA Interference;
RNA, Small Interfering;
genetics;
therapeutic use;
RNA, Viral;
genetics;
Virus Replication
- From:
Chinese Journal of Virology
2012;28(5):527-530
- CountryChina
- Language:Chinese
-
Abstract:
To evaluate the effects of lentivirus-delivered short hairpin RNA (shRNA) on CVB3 infection in an animal model by RNA interference technique, we constructed a recombinant lentivirus expressing shRNA-3753 against the viral genome region 3753-3771, then transduced Lenti-sh3753 into mice infected with CVB3. We evaluated the antiviral ability of lenti-sh3753 by cytopathic effect (CPE), viral plaque assay and histological analysis of mice hearts. The results showed that Lenti-sh3753 exhibited a significant protective effect on cell viability and reduction of viral titers in supernatant of cell culture by specific inhibition on viral replication. Lenti-sh3753 also prolonged the mice survival and limited the viral production in mice hearts. These data proposed that Lenti-sh3753 can effectively inhibit CVB3 infection in a coxsackievirus-induced myocarditis model, suggesting its potential role in prevention and therapy of viral diseases.
