Molecular cloning and expression of bone marrow stromal antigen-2 and detection of its biological activity.
- Author:
Ting-Hong ZHANG
1
;
Xie ZHAO
;
Guang-Ming CAO
;
Zhen-Jie ZHANG
;
Wei-Shan CHANG
Author Information
1. College of Animal Science and Technology, Shandong Agricultural University, Tai'an 271018, China. snowrabbit58@163.com
- Publication Type:Journal Article
- MeSH:
Animals;
Antigens, CD;
genetics;
immunology;
Cell Line;
Chickens;
Cloning, Molecular;
Gene Expression;
Humans;
Influenza in Birds;
immunology;
virology;
Orthomyxoviridae;
physiology;
Porcine Reproductive and Respiratory Syndrome;
immunology;
virology;
Porcine respiratory and reproductive syndrome virus;
physiology;
Swine;
Vesicular Stomatitis;
immunology;
virology;
Vesicular stomatitis Indiana virus;
physiology;
Virus Replication
- From:
Chinese Journal of Virology
2012;28(5):548-553
- CountryChina
- Language:Chinese
-
Abstract:
To clone porcine bone marrow stromal antigen-2 (BST-2) gene, construct its recombinant eukaryotic expression plasmid and induce the expression of the fusion antiviral protein, we amplified BST-2 gene by RT-PCR from the total RNA extracted from PK15 cells. The recombinant expression plasmid pcDNA-BST-2 was constructed and then was transfected into HEK293T cells to expresse the BST-2 fusion protein. Western blot and indirect immunofluorescence assay (IFA) were performed, and the biological activity was detected. The results showed that the construction of recombinant plasmid pcDNA-BST-2 was confirmed by restriction enzyme digestion and sequencing. The expressed product had antiviral activity against Vesicular stomatitis virus (VSV), Avian influenza virus (AIV) and Porcine reproductive and respiratory syndrome virus (PRRSV). In conclusion, the research paves the way for further research on bioactivity assayand antiviral medication.
