Functional analysis of the late expression factor genes of plutella xylostella granulovirus.
- Author:
Lei FAN
1
;
Yuan HU
;
Lu-Lin LI
Author Information
1. Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan 430079, China.
- Publication Type:Journal Article
- MeSH:
Amino Acid Sequence;
Animals;
Gene Expression Regulation, Viral;
Granulovirus;
chemistry;
genetics;
metabolism;
Molecular Sequence Data;
Moths;
virology;
Nucleopolyhedrovirus;
genetics;
metabolism;
Sequence Alignment;
Viral Proteins;
genetics;
metabolism
- From:
Chinese Journal of Virology
2012;28(5):560-566
- CountryChina
- Language:Chinese
-
Abstract:
Plutella xylostella granulovirus (PlxyGV) contains homologs of 15 Autographa californica MNPV (AcMNPV) late expression factor (lef) genes. The prospective products of 14 PlxyGV lef genes (ie-0 is not included) share 13%-53% amino acid similarity with their corresponding homologs of AcMNPV, among which LEF-9, LEF-8 and P47, three subunits of the virus-encoded RNA polymerase, share 49%, 53% and 46% sequence identity, respectively. In this study, an established transient expression system was used to test the ability of the PlxyGV LEFs to activate an AcMNPV vp39 promoter-driven reporter gene in SF9 cells. It was shown that PlxyGV le f-2 replaced the corresponding AcMNPV gene and exhibited partial activity in the context of the remaining set of AcMNPV le fs. PlxyGV LEF-2 was found to contain additional 100aa and 70aa at the C-terminus in comparison with the LEF-2 of other GVs and lepidopteran NPVs respectively.
