Development of SYBR Green I real-time RT-PCR for the detection of Ebola virus.
- Author:
Yang LIU
1
;
Zi-Xue SHI
;
Yu-Kun MA
;
Hao-Ting WANG
;
Zong-Yao WANG
;
Dong-Hua SHAO
;
Jian-Chao WEI
;
Shao-Hui WANG
;
Bei-Bei LI
;
Shui-Ming WANG
;
Xue-Hui LIU
;
Zhi-Yong MA
Author Information
1. Department of Veterinary Public Health, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China.
- Publication Type:Journal Article
- MeSH:
DNA Primers;
chemistry;
genetics;
Ebolavirus;
genetics;
isolation & purification;
Hemorrhagic Fever, Ebola;
virology;
Humans;
Organic Chemicals;
chemistry;
Reverse Transcriptase Polymerase Chain Reaction;
methods
- From:
Chinese Journal of Virology
2012;28(5):567-571
- CountryChina
- Language:Chinese
-
Abstract:
In order to establish a rapid and accurate method for the detection of Ebola virus (EBOV), the primers used in SYBR Green I real-time RT-PCR were designed based on the EBOV NP gene sequences published in GenBank. The SYBR Green I real-time RT-PCR was established and optimized for the detection of EBOV. The EBOV RNA that was transcribed in vitro was used as a template. The sensitivity of this method was found to reach 1.0 x 10(2) copies/microL and the detection range was 10(2) - 10(10). No cross reaction with RNA samples from Marburg virus, Dengue virus, Xinjiang hemorrhagic fever virus, Japanese encephalitis virus, Influenza virus (H1N1 and H3N2) and Porcine reproductive and respiratory syndrome virus E genomic RNA was found. The method would be useful for the detection and monitoring of EBOV in China.
