Clinical characteristics and molecular characteristics of\r\n', u'hepatitis B virus in chronic hepatitis B virus infected patients\r\n', u'with A1899 mutation in precore region\r\n', u'
- Author:
Truong Xuan Bui
;
Ho Thi Thu Pham
;
Phuong Minh Tran
;
Son Truong Nguyen
;
Long Cong Nguyen
;
Trach Khanh Nguyen
;
Yasushi Seo
;
Yoshihiko Yano
- Publication Type:Journal Article
- Keywords:
Molecular characteristics;
hepatitis B virus;
A1899 mutation
- MeSH:
Hepatitis B virus/ growth & development;
physiology;
Hepatitis B;
Chronic/ pathology;
transmission
- From:Journal of Medical Research
2007;47(2):64-68
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
Background: Hepatitis B is an infectious illness caused by hepatitis B virus (HBV) which infects the liver of hominoidea, including humans, and causes an inflammation called hepatitis. Objectives: The aim of study is to clarify clinical features and molecular characteristics of HBV in chronic HBV-infected patients with A 1899 mutation. Subjects and method: HBV genotype, HBV-ONA level, HBeAg and anti-HBe in 29 chronic HBV-infected patients were determined by PCR-RFLP, Real-time PCR and ELISA, respectively. Mutations were analyzed by direct sequencing. Results: Mutations in core-promoter/precore regions of HBV genome can suppress HBeAg secretion and stimulate HBV-ONA replication. The prevalence of hepatocel- lular carcinoma (HCc): 10/29, liver cirrhosis (LC) : 15/29 are significantly higher than that in chronic hepatitis (CH) : 4/29 (P < 0.001). HbeAg seroconversion rate in CH (75%) is higher than that in HCC \r\n', u'(40%) and in LC (53.3%), but not significant (P > 0.05). ALT level is the highest in CH and the lowest in HCC \r\n', u'(P = 0.02), 8/10 (80%) HCC patients have normal range of ALT. HBV-ONA level in HCC and in LC is significantly higher than that in CH (P = 0.024). The emerging of A 1899 is often accompanied by C/G1753 mutation (37.9%) and dual core-promoter mutation T1762A1764 (79.3%). Conclusion: A1899 mutation can play a role in the pathogenesis of liver diseases in chronic HBV-infected Vietnamese.\r\n', u'
