Comparison of different molecular assays for the rapid detection of enterovirus 71 (EV71).
- Author:
Hai-Yan WEI
1
;
Xue-Yong HUANG
;
Yu-Ling XU
;
Hong MA
;
Hao-Min CHEN
;
Bian-Li XU
Author Information
1. Henan Center for Disease Prevention and Control, Zhengzhou 450016, China. msweihaiyan@yahoo.com.cn
- Publication Type:Journal Article
- MeSH:
Enterovirus A, Human;
classification;
genetics;
isolation & purification;
Enterovirus Infections;
diagnosis;
virology;
Humans;
Real-Time Polymerase Chain Reaction;
methods;
Reverse Transcriptase Polymerase Chain Reaction;
methods;
Sensitivity and Specificity
- From:
Chinese Journal of Virology
2012;28(6):670-674
- CountryChina
- Language:Chinese
-
Abstract:
Molecular detection of enterovirus (EV)71 RNA based on PCR methods is a quick and sensitive approach. At present, different PCR-based methods for EV71 RNA detection are available, but comparisons of results obtained using different approaches are limited. This study is to compare the analytical sensitivity and specificity of different real-time reverse transcription-polymerase chain reaction (rRT-PCR) and conventional reverse transcription-polymerase chain reaction (cRT-PCR) assays for enterovirus and EV71 detection, Altogether, three rRT-PCR assays and one cRT-PCR assay targeting the 5'UTR gene for universal detection of enterovirus; two rRT-PCR assays andone cRT-PCR assay targeting the VP1 gene for specific detection of EV 71 were examined. All assays showed good specificity. The detection sensitivity ranged from 8.19 x 10 to 8.19 x 10(5) copy equivalents. In general, rRT-PCR assays were more sensitive than cRT-PCR assays. All rRT-PCR assays showed 100% sensitivity for clinical specimens.
