Development of real-time fluorescent quantitative PCR method for detecting Bombyx mori cytoplasmic polyhedrosis virus.
- Author:
Ping WU
1
;
He-Ying QIAN
;
Xi-Jie GUO
;
Long LI
;
Tao CHEN
Author Information
1. Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang 212018, China. wp4114@163.com
- Publication Type:Journal Article
- MeSH:
Animals;
Bombyx;
virology;
Fluorescence;
Real-Time Polymerase Chain Reaction;
methods;
Reoviridae;
genetics;
isolation & purification
- From:
Chinese Journal of Virology
2013;29(1):12-16
- CountryChina
- Language:Chinese
-
Abstract:
A pair of specific primers were designed according to the published 118 bp conserved sequence of polyhedrin gene of Bombyx mori cytoplasmic polyhedrosis virus (BmCPV) in GenBank and a serial dilutions of a recombinant plasmid were prepared and used to generate standard curves, to establish a real-time fluorescent quantitative PCR method for detection of BmCPV. The results showed that the linear relationship between virus concentration (X) and Ct (Y) was Y = -3. 582 lgX + 38.748 with the correlation coefficient R2 = 0999. The method was very sensitive, specific and reproducible. It can be applied in the rapid detection of BmCPV and the prevalence investigation of this disease.
