Utility of high throughput sequencing technology in analyzing the terminal sequence of caudovirales bacteriophage genome.
- Author:
Sha-Sha LI
1
;
Hang FAN
;
Xiao-Ping AN
;
Hua-Hao FAN
;
Huan-Huan JIANG
;
Zhi-Qiang MI
;
Yi-Gang TONG
Author Information
1. Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China. lzsjsz@yahoo.com.cn
- Publication Type:Journal Article
- MeSH:
Caudovirales;
genetics;
Computational Biology;
Genome, Viral;
High-Throughput Nucleotide Sequencing;
methods
- From:
Chinese Journal of Virology
2013;29(1):39-43
- CountryChina
- Language:Chinese
-
Abstract:
To confirm the hypothesis that the high frequency sequences of high throughput sequencing are the terminal sequences of the bacteriophage genome. An adaptor of specific sequence was linked to the end of the bacteriophage T3 genomic DNA, which was then subject to high throughput sequencing; as a control, the same T3 genomic DNA without adaptor was also analyzed by high throughput sequencing. The sequencing results were examined with bioinformatics software. Similar high throughput sequencing technique was applied to analyze the genomic sequence of N4-like bacteriophage IME11. Bioinformatics study showed that the sequences tagged with adaptors were consistent with the high frequency sequences without adaptor labeling. Our analysis also indicated that the end of the T4-like phage genome had specific sequences instead of random sequences, disagreeing with the previous assertion. Evidences were provided that N4-like bacteriophage had a particular terminal sequence: the left end of the genome was unique while the right end was permuted. The high throughput sequencing technique was convenient and practical to be used to simultaneously detect the terminal sequence and the complete sequence of bacteriophage genome.
