Detection methods for retroviruses and reverse transcriptase: commentary.
- Author:
Rong MA
1
;
Ying-Jie GAO
;
Xiao-Lan CUI
Author Information
1. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. haijiaosky@yahoo.com.cn
- Publication Type:Journal Article
- MeSH:
Enzyme-Linked Immunosorbent Assay;
Microscopy, Electron;
Polymerase Chain Reaction;
RNA-Directed DNA Polymerase;
analysis;
Retroviridae;
isolation & purification
- From:
Chinese Journal of Virology
2013;29(1):92-96
- CountryChina
- Language:Chinese
-
Abstract:
Retroviruses are often used as a carrier for expression of target protein or chimeric target gene. Although non-infectious viruses are selected in the laboratory, it does not exclude harms form these viruses. The monitoring and detection of retroviruses has a very important significance. Reverse transcriptase activity is an important indicator of retrovirus replication. Herein, methods for detection of retroviruses and reverse transcriptase are reviewed for further research references.