Establishment and identification of A new HPV positive esophageal cancer cell line.
- Author:
Jin-Tao LI
1
;
Fu-You ZHOU
;
Wen-Ping DONG
;
Li-Dong WANG
;
Yi ZENG
Author Information
1. The College of Life Science and Bio-engineering, Beijing University of Technology, Beijing 100124,China.
- Publication Type:Journal Article
- MeSH:
Aged;
Animals;
Carcinoma, Squamous Cell;
virology;
Cell Proliferation;
China;
Esophageal Neoplasms;
virology;
Female;
Human papillomavirus 18;
genetics;
isolation & purification;
Humans;
Male;
Mice;
Mice, SCID;
Papillomavirus Infections;
virology;
Tumor Cells, Cultured;
cytology;
virology;
Tumor Virus Infections;
virology
- From:
Chinese Journal of Virology
2013;29(2):119-125
- CountryChina
- Language:Chinese
-
Abstract:
In order to explore the relationship between human papilloma virus ( HPV) and upper gastrointestinal cancer(esophageal cancer), An esophageal squamous cell carcinoma(ESCC) tissue was obtained from a 76 year old Chinese female patient from Anyang city, a high-incidence area for esophageal cancer, in China. Transplanted tumor was formed through direct SCID mouse tumorigenicity experiment and cultured monolayer cells were obtained after several passages and screenings Immunofluorescence test, cell growth curve, soft agar assay, chromosome analysis and tissues HE staining were also performed to confirm the epithelial cell origin. Cell DNA STR typing results showed that no three alleles was observed,indicating no contamination of human cells. DNA analysis revealed the presence of HPV type 18 DNA in this cell line. DOLINK test found the E6 protein expression of HPV virus. We concluded that the established cell line is a new esophageal squamous cell-origincarcinoma cell line with HPV DNA positive and expression of viral oncoprotein. It provides new cytologic material for performing etiology studies on the occurrence and development of esophageal cancer.
