The establishment of a new resequencing pathogen microarray-based assay for detecting unexplained respiratory tract infections.
- Author:
Hong-Wei SHEN
1
;
Jin LI
;
Miao WANG
;
Chen ZHANG
;
Ji WANG
;
Kai NIE
;
Meng-Jie YANG
;
Yi ZHANG
;
Wen-Jie TAN
;
Xue-Jun MA
Author Information
1. National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China. feichuan1989@163.com
- Publication Type:Journal Article
- MeSH:
Child;
Child, Preschool;
Female;
Humans;
Male;
Oligonucleotide Array Sequence Analysis;
methods;
Respiratory Tract Infections;
diagnosis;
virology;
Sensitivity and Specificity;
Viruses;
classification;
genetics;
isolation & purification
- From:
Chinese Journal of Virology
2013;29(2):137-142
- CountryChina
- Language:Chinese
-
Abstract:
Resequencing Pathogen Microarray (RPM) is a new pathogen detection and identification technology based on DNA microarray. In order to apply RPM in the detection of unexplained infection and as a result, to improve the emergency response capacity, a new RPM-based respiratory pathogens detection assay was developed to simultaneously detect 19 common respiratory viruses, 9 influenza A viruses (Flu A),11 human rhinoviruses(HRV), 28 enteroviruses and 18 rare respiratory viruses. The specificity of multiplex system was examined by confirmed positive specimens for 16 common respiratory virus. The sensi-tivity was evaluated by serial ten-fold dilutions of plasmids or in vitro-transcribed RNA. RPM could detect and differentiate 16 virus types/subtypes at 10 - 1 000 copies/reaction level. Nucleic acids of 8 throat swabs with unexplained respiratory tract infections were pooled and detected by the new assay. The RPM result was verified by common PCR followed by sequencing as well as PLEX-ID (Abbott). Except for a false-positive of PIV1, no difference among the three assays was found. These results indicate the assay based on the new RPM is a highly sensitive, high throughput test for the detection of respiratory virus infections, which is significant for the management of emergent and epidemic infectious disease.
