The analysis of hantavirus S gene in Apodemus agrarius in Changbai area.
- Author:
Qing-Li YAN
1
;
Peng-Feil YANG
;
Li-Jun SHAO
;
Yong-Xian LIU
;
Yun PU
;
Xiao-Long ZHANG
;
Xiao-Mei CAO
;
Tian-Yu GUO
;
Li-Si YAO
Author Information
1. Chinese Academy of Inspection and Quarantine, Beijing 100123,China. yql0610@163.com
- Publication Type:Journal Article
- MeSH:
Animals;
Base Sequence;
China;
epidemiology;
DNA, Complementary;
chemistry;
genetics;
Disease Reservoirs;
virology;
Genotype;
Hantavirus;
classification;
genetics;
isolation & purification;
Hantavirus Infections;
epidemiology;
veterinary;
virology;
Lung;
virology;
Murinae;
virology;
Phylogeny;
RNA, Viral;
genetics;
Reverse Transcriptase Polymerase Chain Reaction;
Rodent Diseases;
virology;
Sequence Analysis, DNA;
Viral Proteins;
genetics
- From:
Chinese Journal of Virology
2013;29(4):382-385
- CountryChina
- Language:Chinese
-
Abstract:
To gain more insights into epidemiologic characteristics and genotype of hantavirus in Apodemus agrarius in Changbai Area. Complete hantavirus S segment sequences were amplified by RT-PCR and sequenced. The phylogenetic trees were constructed for analysis of genetic characters of hantavirus. A total of 58 Apodemus agrarius were trapped in the epidemic areas, and complete hantavirus S segment sequences were obtained from 4 lung samples of these rodents (6. 90%0). Phylogenetic analysis of the four S segment sequences indicated that all viruses isolated from Apodemu sagrarius were closely related to genotype 6 of Hantaan virus (95. 8%-96. 3%, nucleotide identity; 98. 6%-99. 5%, amino acid identity), all of them had a specific S387 different from other genotypes of Hantaan virus.
