Inhibition of proliferation of H5N1 subtype AIV in CEF by chemosynthetic siRNA.
- Author:
Ru-Shu LI
1
;
Dan YU
;
Bao-Zheng LUO
;
Qing-Ru BO
;
Hai-Nie XU
;
Cai-Hua SHA
;
Xiu-Yun LIAO
Author Information
1. Zhuhai Epidemic Prevention and Supervision Center for Animals and Plants, Zhuhai 519015, China. lirushu@163.com
- Publication Type:Journal Article
- MeSH:
Actins;
genetics;
Animals;
Chick Embryo;
DNA Primers;
genetics;
Fibroblasts;
virology;
Hemagglutination;
Hemagglutinin Glycoproteins, Influenza Virus;
genetics;
Hemagglutinins;
genetics;
Humans;
Influenza A Virus, H5N1 Subtype;
genetics;
growth & development;
physiology;
RNA Interference;
RNA Replicase;
genetics;
RNA, Small Interfering;
chemical synthesis;
genetics;
RNA-Binding Proteins;
genetics;
Real-Time Polymerase Chain Reaction;
Specific Pathogen-Free Organisms;
Transfection;
Viral Core Proteins;
genetics;
Viral Proteins;
genetics;
Virus Replication
- From:
Chinese Journal of Virology
2013;29(4):386-391
- CountryChina
- Language:Chinese
-
Abstract:
In order to study the proliferation inhibition effect of H5N1 subtype avian influenza virus (AIV) with small interfere RNA (siRNA), a total of 4 siRNAs were designed in accordance with the NP and PA genes of H5N1 subtype AIV, the siRNAs were then transfected to chicken embryo fibroblast(CEF), CEF was infected with H5N1 subtype AIV after 6 hrs. Virus titer of cell supernatant was tested at 16-56hrs post infection, and pathological changes of the cells was observed; mRNA levels of NP, PA, HA and p13-actin gene were tested at 36hrs post infection. The results showed that these 4 siRNAs could inhibit the prolif-eration of H5N1 subtype AIV in CEF in varying degrees, and one siRNA targeting PA was best per-formed. The experimental results also showed that the inhibition effect was decreased with the time prolonged. This research provides a basis for further studying RNAi on AIV prevention and control.
